option --auto An appropriately strategy is automatically selected according to data size (Beta). default: off --globalpair All pairwise alignments are computed with the FFT approximation. default: off --localpair All pairwise alignments are computed by the Smith-Waterman algorithm. default: off --memsave Memory saving. default: auto --op n n = gap opening penalty for group-to-group alignment. n = 1.53 by default. --ep n n = offset value, which works like gap extension penalty, for group-to-group alignment. n = 0.123 by default. --lop n n = gap opening penalty for local alignment. n = -2 by default. --lep n n = offset value, which works like gap extension penalty for local alignment. n = +0.1 by default. --lexp n n = gap extension penalty for local alignment. n = -0.1 by default. --bl n Scoring matrix = BLOSUM n, n = 30, 45, 62 (default), 80. --jtt n Scoring matrix = JTT n PAM. --nuc Sequence type = nucleotide. default: auto --amino Sequence type = amino acid. default: auto --retree n n is the number of tree re-buiding in progressive method. See the paper for details. n = 2 (default), 3, or so --maxiterate n n is the maximum number of iterative refinement. default: 0 --fft FFT is enabled at progressive alignment stage. See the paper for details. --nofft FFT is disabled. --clustalout Output format: clustal format. default: fasta format --reorder Output order: aligned. default: same as input order --quiet Do not report progress.